Selection and validation of potato candidate genes for maturity corrected resistance to Phytophthora infestans based on differential expression combined with SNP association and linkage mapping.

Late blight of potato (Solanum tuberosum L.) caused by the oomycete Phytophthora infestans (Mont.) de Bary, is one of the most important bottlenecks of potato production worldwide. Cultivars with high levels of durable, race unspecific, quantitative resistance are part of a solution to this problem. However, breeding for quantitative resistance is hampered by the correlation between resistance and late plant maturity, which is an undesirable agricultural attribute. The objectives of our research are (i) the identification of genes that condition quantitative resistance to P. infestans not compromised by late plant maturity and (ii) the discovery of diagnostic single nucleotide polymorphism (SNP) markers to be used as molecular tools to increase efficiency and precision of resistance breeding. Twenty two novel candidate genes were selected based on comparative transcript profiling by SuperSAGE (serial analysis of gene expression) in groups of plants with contrasting levels of maturity corrected resistance (MCR). Reproducibility of differential expression was tested by quantitative real time PCR and allele specific pyrosequencing in four new sets of genotype pools with contrasting late blight resistance levels, at three infection time points and in three independent infection experiments. Reproducibility of expression patterns ranged from 28 to 97%. Association mapping in a panel of 184 tetraploid cultivars identified SNPs in five candidate genes that were associated with MCR. These SNPs can be used in marker-assisted resistance breeding. Linkage mapping in two half-sib families (n = 111) identified SNPs in three candidate genes that were linked with MCR. The differentially expressed genes that showed association and/or linkage with MCR putatively function in phytosterol synthesis, fatty acid synthesis, asparagine synthesis, chlorophyll synthesis, cell wall modification, and in the response to pathogen elicitors

The historical role of species from the Solanaceae plant family in genetic research

KEY MESSAGE: This article evaluates the main contributions of tomato, tobacco, petunia, potato, pepper and eggplant to classical and molecular plant genetics and genomics since the beginning of the twentieth century. ABSTRACT: Species from the Solanaceae family form integral parts of human civilizations as food sources and drugs since thousands of years, and, more recently, as ornamentals. Some Solanaceous species were subjects of classical and molecular genetic research over the last 100 years. The tomato was one of the principal models in twentieth century classical genetics and a pacemaker of genome analysis in plants including molecular linkage maps, positional cloning of disease resistance genes and quantitative trait loci (QTL). Besides that, tomato is the model for the genetics of fruit development and composition. Tobacco was the major model used to establish the principals and methods of plant somatic cell genetics including in vitro propagation of cells and tissues, totipotency of somatic cells, doubled haploid production and genetic transformation. Petunia was a model for elucidating the biochemical and genetic basis of flower color and development. The cultivated potato is the economically most important Solanaceous plant and ranks third after wheat and rice as one of the world’s great food crops. Potato is the model for studying the genetic basis of tuber development. Molecular genetics and genomics of potato, in particular association genetics, made valuable contributions to the genetic dissection of complex agronomic traits and the development of diagnostic markers for breeding applications. Pepper and eggplant are horticultural crops of worldwide relevance. Genetic and genomic research in pepper and eggplant mostly followed the tomato model. Comparative genome analysis of tomato, potato, pepper and eggplant contributed to the understanding of plant genome evolution

Fabry-Perot Measurements of the Dynamics of Globular Cluster Cores: M15 (NGC~7078)

We report the first use of the Rutgers Imaging Fabry-Perot Spectrophotometer to study the dynamics of the cores of globular clusters. We have obtained velocities for cluster stars by tuning the Fabry-Perot to take a series of narrow-band images at different wavelengths across one of the Na D (5890 AA) absorption lines. Measuring the flux in every frame yields a short portion of the spectrum for each star simultaneously. This proves to be a very efficient method for obtaining accurate stellar velocities; in crowded regions we are able to measure hundreds of velocities in 3-4 hours of observing time. We have measured velocities with uncertainties of less than 5 km/s for 216 stars within 1.5' of the center of the globular cluster M15 (NGC 7078). The paper is a uuencoded compressed postscript file

Open Water Propeller Tests in Four Operating Modes

http://deepblue.lib.umich.edu/bitstream/2027.42/96584/1/39015087358282.pd

On Objective Measures of Rule Surprisingness

Most of the literature argues that surprisingness is an inherently subjective aspect of the discovered knowledge, which cannot be measured in objective terms. This paper departs from this view, and it has a twofold goal: (1) showing that it is indeed possible to define objective (rather than subjective) measures of discovered rule surprisingness; (2) proposing new ideas and methods for defining objective rule surprisingness measures

A 20 Thousand Solar Mass Black Hole in the Stellar Cluster G1

We present the detection of a 2.0(+1.4,-0.8)x10^4 solar mass black hole (BH) in the stellar cluster G1 (Mayall II), based on data taken with the Space Telescope Imaging Spectrograph onboard the Hubble Space Telescope. G1 is one of the most massive stellar clusters in M31. The central velocity dispersion (25 kms) and the measured BH mass of G1 places it on a linear extrapolation of the correlation between BH mass and bulge velocity dispersion established for nearby galaxies. The detection of a BH in this low-mass stellar system suggests that (1) the most likely candidates for seed massive BHs come from stellar clusters, (2) there is a direct link between massive stellar clusters and normal galaxies, and (3) the formation process of both bulges and massive clusters is similar due to their concordance in the M_BH/sigma relation. Globular clusters in our Galaxy should be searched for central BHs.Comment: 4 pages, accepted in The Astrophysical Journal Letters, October 200

Investigating Fatigue Performance on the Foamed Asphalt Specimens Generated Using Different Foam Properties

An evaluation of fatigue resistance for foamed asphalt mixture is very demanding since the binder is not continuously distributed on the aggregate surface and this mixtures contains water, the content of which dramatically affects the mechanical properties. This paper discusses the results of laboratory fatigue testingon the foamed asphalt mixtures in which the specimens are generated using three different foamed bitumen properties. Foamed bitumen as the binder was produced at three different foaming water content (FWC) at a temperature of 180oC using a 70/100 pen. The aggregates were mechanically mixed with foamed bitumen using a Hobart mixer. The resulting mixtures were then compacted using a gyratory compactor to generatespecimen with diameter of 100 mm. The specimens were fatigue tested at various stress levels at a temperature of 20oC following a curing period of 3 days at 40oC. Overall, fatigue performance of foamed asphalt can be identified based upon both stress and strain for mixtures produced at FWC 1%, 5%, and 10%